Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutamate-cysteine ligase, catalytic subunit | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GCLC |
Protein |
Glutamate–cysteine ligase catalytic subunit |
Uniprot ID |
P48506 |
Function |
|
Tissue Specificity |
|
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the glutamate–cysteine ligase type 3 family. |
Aliases |
EC 6.3.2.2 antibody|Gamma ECS antibody|Gamma GCS antibody|Gamma glutamylcysteine synthetase antibody|Gamma-ECS antibody|Gamma-glutamylcysteine synthetase antibody|GCL antibody|GCLC antibody|GCLM antibody|GCS antibody|GCS Heavy Chain antibody|GLCL antibody|GLCLC antibody|GLCLR antibody|Glutamate cysteine ligase catalytic subunit antibody|Glutamate cysteine ligase (gamma glutamylcysteine synthetase) regulatory (30.8kD) antibody|Glutamate cysteine ligase catalytic subunit antibody|Glutamate cysteine ligase modifier subunit antibody|Glutamate cysteine ligase regulatory protein antibody|Glutamate cysteine ligase regulatory subunit antibody|Glutamate–cysteine ligase catalytic subunit antibody|GSC light chain antibody|GSH1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GCLC antibody, ASA-B0772, Western blotting
All lanes: Anti GCLC (ASA-B0772) at 0.5ug/ml
WB: Recombinant Human GCLC Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti GCLC (ASA-B0772) at 0.5ug/ml
WB: Recombinant Human GCLC Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti- GCLC antibody, ASA-B0772, Western blotting
All lanes: Anti GCLC (ASA-B0772) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: HELA Cell Lysate at 40ug
Lane 4: PC-12 Whole Cell Lysate at 40ug
Lane 5: NRK Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Predicted bind size: 72KD
Observed bind size: 72KD
All lanes: Anti GCLC (ASA-B0772) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 3: HELA Cell Lysate at 40ug
Lane 4: PC-12 Whole Cell Lysate at 40ug
Lane 5: NRK Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Predicted bind size: 72KD
Observed bind size: 72KD
Anti- GCLC antibody, ASA-B0772, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
