Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| eukaryotic translation initiation factor 2 alpha kinase 4 | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2(868-886aa ATDHLAFTAEGKQDDQAGD), different from the related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
EIF2AK4 |
Protein |
Eukaryotic translation initiation factor 2-alpha kinase 4 |
Uniprot ID |
Q9QZ05 |
Function |
Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. |
Tissue Specificity |
Expressed in liver (at protein level). Widely expressed, with the highest levels in the liver and the brain. Isoform 4 is expressed in brain followed by testis and liver and isoform 3 is expressed only in brain. |
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. |
Aliases |
E2AK4_HUMAN antibody|Eif2ak4 antibody|Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody|Eukaryotic translation initiation factor 2-alpha kinase 4 antibody|GCN2 antibody|GCN2 eIF2alpha kinase antibody|GCN2 like protein antibody|GCN2-like protein antibody|KIAA1338 antibody|MGCN2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GCN2 antibody, ASA-B0774, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Mouse EIF2AK4, 35.3KD(162aa tag+ E801-R954)
Lane 1: Recombinant Mouse EIF2AK4 Protein 10ng
Lane 2: Recombinant Mouse EIF2AK4 Protein 5ng
Lane 3: Recombinant Mouse EIF2AK4 Protein 2.5ng
Lane 4: Recombinant Mouse EIF2AK4 Protein 1.25ng
Recombinant Protein Detection Source: E.coli derived -recombinant Mouse EIF2AK4, 35.3KD(162aa tag+ E801-R954)
Lane 1: Recombinant Mouse EIF2AK4 Protein 10ng
Lane 2: Recombinant Mouse EIF2AK4 Protein 5ng
Lane 3: Recombinant Mouse EIF2AK4 Protein 2.5ng
Lane 4: Recombinant Mouse EIF2AK4 Protein 1.25ng
