Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glial fibrillary acidic protein | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GFAP |
Protein |
Glial fibrillary acidic protein |
Uniprot ID |
P14136 |
Function |
GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
Tissue Specificity |
Expressed in cells lacking fibronectin. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the intermediate filament family. |
Aliases |
Astrocyte antibody|FLJ42474 antibody|FLJ45472 antibody|GFAP antibody|GFAP_HUMAN antibody|Glial Fibrillary Acidic Protein antibody|Intermediate filament protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GFAP antibody, ASA-B0783–1.jpg
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti-GFAP antibody, ASA-B0783–2.jpg
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-GFAP antibody, ASA-B0783–3.jpg
IHC(P): Human meningioma Tissue
IHC(P): Human meningioma Tissue
