GFAP Antibody (monoclonal)

GFAP Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B0781 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
glial fibrillary acidic protein Monoclonal IgG Mouse Human, Pig, Rat IHC-P, IHC-F, WB Ascites

Immunogen
GFAP from pig spinal cord.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 GFAP

Protein

 Glial fibrillary acidic protein

Uniprot ID

 Human(P14136), Mouse(P03995), Rat(P47819)

Function

 GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Tissue Specificity

Expressed in cells lacking fibronectin.

Sub-cellular localization

 Cytoplasm.

Sequence Similarities

 Belongs to the intermediate filament family.

Aliases

 FLJ45472|intermediate filament protein

Application Details

Application Concentration* Species Validated Using**
Western blot 0.5-1μg/ml Human, pig, rat AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.4-1μg/ml Human, pig, rat AssaySolutio’s IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Human, pig, rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- GFAP antibody, ASA-B0781, IHC(P)
IHC(P): Rat Brain Tissue

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