Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| GDNF family receptor alpha 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GFRA1 |
Protein |
GDNF family receptor alpha-1 |
Uniprot ID |
P56159 |
Function |
Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity). |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane ; Lipid-anchor, GPI-anchor . |
Sequence Similarities |
|
Aliases |
GDNF Family Receptor Alpha 1 antibody|GDNF R antibody|GDNF RA antibody|GDNF receptor alpha antibody|GDNFR alpha 1 antibody|GDNFR alpha antibody|GDNFR antibody|GDNFRA antibody|GDNFRalpha antibody|GFR alpha 1 antibody|GFR alpha1 antibody|GFR-ALPHA-1 antibody|GFRA 1 antibody|GFRA1 antibody|GFRalpha1 antibody|Glial Cell Line DErived Neurotrophic Factor Receptor Alpha antibody|Glial cell line-derived neurotrophic factor receptor alpha antibody|GPI linked anchor protein antibody|GPI-linked anchor protein antibody|MGC23045 antibody|PI linked cell surface accessory protein antibody|RET 1L antibody|RET ligand 1 antibody|RET1L antibody|RETL 1 antibody|RETL1 antibody|TGF beta related neurotrophic factor receptor 1 antibody|TGF-beta related neurotrophic factor receptor 1 antibody|TRNR 1 antibody|TRNR1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GFRA1 antibody, ASA-B0784, Western blotting
All lanes: Anti GFRA1 (ASA-B0784) at 0.5ug/ml
WB: Recombinant Human GFRA1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti GFRA1 (ASA-B0784) at 0.5ug/ml
WB: Recombinant Human GFRA1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- GFRA1 antibody, ASA-B0784, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti- GFRA1 antibody, ASA-B0784, Western blotting
All lanes: Anti GFRA1 (ASA-B0784) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 51KD
Observed bind size: 51KD
All lanes: Anti GFRA1 (ASA-B0784) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 51KD
Observed bind size: 51KD
