Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MGKSASKQFH NEVLKAHNEY RQKHGVPPLK LCKNLNREAQ QYSEALASTR ILKHSPESSR GQCGENLAWA SYDQTGKEVA DRWYSEIKNY NFQQPGFTSG TGHFTAMVWK NTKKMGVGKA SASDGSSFVV ARYFPAGNVV NEGFFEENVL PPKK. |
| Antibody Properties | Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Description | GLIPR2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (1-154) & having a molecular mass of 19.3 kDa.The GLIPR2 is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The GLIPR2 1mg/ml protein contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1M DTT and 10% Glycerol. |
| Physical Appearence | Sterile filtered colorless solution. |
| Protein Background | GLIPR2 is linked to plant pathogenesis-related (PR-1) proteins, which are upregulated in reaction to pathogen attack. GLIPR2 is localized within lipid-enriched microdomains on the cytosolic side of the endomembrane system. GLIPR2 is closely attached to membranes and lacks the cytosol, though it lacks a membrane-spanning domain. |
| Protein content | By direct ELISA, 1 : 20,000 dilution will yield >1 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Stability | Store GLIPR2 at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Synonyms | GAPR-1, Golgi-associated plant pathogenesis-related protein 1, GAPR1, GLIPR-2, Golgi-associated PR-1 protein. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
