Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutathione peroxidase 2(gastrointestinal) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Glutathione Peroxidase 2(44-60aa TRDFTQLNELQCRFPRR), different from the related mouse sequence by two amino acids and from related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GPX2 |
Protein |
Glutathione peroxidase 2(GPx-2) |
Uniprot ID |
P18283 |
Function |
Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. |
Tissue Specificity |
Mostly in liver and gastrointestinal tract, not found in heart or kidney. |
Sub-cellular localization |
Cytoplasm. Note: Mainly cytoplasmic. |
Sequence Similarities |
Belongs to the glutathione peroxidase family. |
Aliases |
Gastrointestinal glutathione peroxidase antibody|GI GPx antibody|Glutathione peroxidase 2(gastrointestinal) antibody|Glutathione peroxidase 2 antibody|Glutathione peroxidase gastrointestinal antibody|Glutathione peroxidase related protein 2 antibody|Glutathione peroxidase-gastrointestinal antibody|Glutathione peroxidase-related protein 2 antibody|GPRP antibody|GPRP-2 antibody|GPx 2 antibody|GPx-2 antibody|GPx-GI antibody|GPX2 antibody|GPX2_HUMAN antibody|GSHPx 2 antibody|GSHPx GI antibody|GSHPx-2 antibody|GSHPx-GI antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Glutathione Peroxidase 2 antibody, ASA-B0803, Western blotting
Lane 1: Rat liver Tissue Lysate
Lane 2: SW620 Cell Lysate
Lane 1: Rat liver Tissue Lysate
Lane 2: SW620 Cell Lysate
Anti-Glutathione Peroxidase 2 antibody, ASA-B0803, ICC
ICC: HELA Cell
ICC: HELA Cell
Anti-Glutathione Peroxidase 2 antibody, ASA-B0803, ICC
ICC: HELA Cell
ICC: HELA Cell
