Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutathione S-transferase theta 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Glutathione S Transferase theta 1(96-112aa DEYLAWQHTTLRRSCLR), identical to the related rat sequence, and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GSTT1 |
Protein |
Glutathione S-transferase theta-1 |
Uniprot ID |
P30711 |
Function |
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. |
Tissue Specificity |
Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the GST superfamily. Theta family. |
Aliases |
EC 2.5.1.18 antibody|Glutathione S transferase 5 antibody|Glutathione S transferase theta 1 antibody|Glutathione S-transferase theta-1 antibody|Glutathione transferase T1 1 antibody|Glutathione transferase T1-1 antibody|GST 5 5 antibody|GST CL1 antibody|GST class theta 1 antibody|GST class-theta-1 antibody|GSTT1 antibody|GSTT1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Glutathione S Transferase theta 1 antibody, ASA-B0804, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 1: COLO320 Cell Lysate
Lane 2: SW620 Cell Lysate
