Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| colony stimulating factor 2(granulocyte-macrophage) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human GM-CSF(63-81aa MFDLQEPTCLQTRLELYKQ). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CSF2 |
Protein |
Granulocyte-macrophage colony-stimulating factor(GM-CSF) |
Uniprot ID |
P04141 |
Function |
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the GM-CSF family. |
Aliases |
Burst Promoting Activity antibody|CMCSF antibody|Colony stimulating factor 2(granulocyte-macrophage) antibody|Colony Stimulating Factor 2 antibody|Colony stimulating factor antibody|Colony-stimulating factor antibody|CSF 2 antibody|CSF Alpha antibody|CSF antibody|CSF2 antibody|CSF2_HUMAN antibody|Eosinophil Colony Stimulating Factor antibody|GM-CSF antibody|GMCSF antibody|Granulocyte Macrophage Colony Stimulating Factor antibody|Granulocyte-macrophage colony-stimulating factor antibody|MGC131935 antibody|MGC138897 antibody|Molgramostin antibody|Pluripoietin Alpha antibody|Sargramostim antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GM-CSF antibody, ASA-B0805, Western blotting
All lanes: Anti GM-CSF (ASA-B0805) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HT1080Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 35KD
All lanes: Anti GM-CSF (ASA-B0805) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HT1080Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 35KD
