Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| granzyme A(granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Granzyme A(175-190aa DRKVCNDRNHYNFNPV). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GZMA |
Protein |
Granzyme A |
Uniprot ID |
P12544 |
Function |
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after ‘Lys-31’ and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after ‘Lys-189’, which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA. |
Tissue Specificity |
|
Sub-cellular localization |
Isoform alpha: Secreted. Cytoplasmic granule. |
Sequence Similarities |
Belongs to the peptidase S1 family. Granzyme subfamily. |
Aliases |
CTL tryptase antibody|CTLA3 antibody|Cytolytic t cell and natural killer cell specific trypsin like serine protease antibody|Cytotoxic T lymphocyte associated serine esterase 3 antibody|Cytotoxic T lymphocyte proteinase 1 antibody|Cytotoxic T-lymphocyte proteinase 1 antibody|Fragmentin 1 antibody|Fragmentin-1 antibody|GRAA_HUMAN antibody|Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3 antibody|Granzyme A(Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease) antibody|Granzyme A(granzyme 1, cytotoxic T lymphocyte associated serine esterase 3) antibody|Granzyme A antibody|Granzyme A precursor antibody|Granzyme-1 antibody|GZMA antibody|H factor antibody|Hanukah factor serine protease antibody|Hanukkah factor antibody|HF antibody|HFSP antibody|TSP1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Granzyme A antibody, ASA-B0810, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
Anti-Granzyme A antibody, ASA-B0810, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
