Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| granzyme B(granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Granzyme B(168-184aa QEDRKCESDLRHYYDST). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GZMB |
Protein |
Granzyme B |
Uniprot ID |
P10144 |
Function |
This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasmic granule. |
Sequence Similarities |
Belongs to the peptidase S1 family. Granzyme subfamily. |
Aliases |
C11 antibody|Cathepsin G like 1 antibody|Cathepsin G-like 1 antibody|CCPI antibody|CGL 1 antibody|CGL1 antibody|CSP B antibody|CSPB antibody|CTLA-1 antibody|CTLA1 antibody|CTSGL1 antibody|Cytotoxic serine protease B antibody|Cytotoxic T lymphocyte associated serine esterase 1 antibody|Cytotoxic T lymphocyte proteinase 2 antibody|Cytotoxic T-lymphocyte proteinase 2 antibody|Fragmentin 2 antibody|Fragmentin-2 antibody|GRAB_HUMAN antibody|Granzyme 2 antibody|Granzyme B(granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody|Granzyme B antibody|Granzyme-2 antibody|GranzymeB antibody|GRB antibody|Gzmb antibody|Hlp antibody|Human lymphocyte protein antibody|Lymphocyte protease antibody|Protease, serine, B antibody|SECT antibody|T cell serine protease 1-3E antibody|T-cell serine protease 1-3E antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Granzyme B antibody, ASA-B0811, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HEPA Cell Lysate
Lane 1: SMMC Cell Lysate
Lane 2: HEPA Cell Lysate
