Description
Data Sheet
| Concentration | Polyclonal antibody against GST may be used for the capturing of GST fusion proteins using various immunochemical assays including immunobotting, dot blotting, immunoprecipitation, ELISA and immunohistochemistry (immunofluorescence or immuno-enzymatic staining). |
| Description | Growth Hormone Rabbit Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21774 Dalton. GH is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
| Note | Western Blotting; 1/2,000. ELISA; 1/40,000. |
| Product Description | Polyclonal antiserum against GST recognizes recombinant GST. It reacts specifically to a variety of GST fusion proteins used as targets in immunoblotting experiments. It also immunoprecipitates the target proteins efficiently. This antibody is reactive with immobilized native antigens on ELISA plates as well. |
| Protein Background | GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. |
| Purity | Greater than 97.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Recommended Dilution | 1mg/1ml. |
| Solubility | It is recommended to reconstitute the lyophilized Growth Hormone Rabbit in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Growth Hormone Rabbit although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GH Rabbit should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin. |
