Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| tuberous sclerosis 1 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Hamartin recombinant protein (Position: D686-Y884). Human Hamartin shares 96% and 95% amino acid (aa) sequences identity with mouse and rat Hamartin, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TSC1 |
Protein |
Hamartin |
Uniprot ID |
Q92574 |
Function |
In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. |
Tissue Specificity |
Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
Hamartin antibody|kiaa0243 antibody|LAM antibody|TSC antibody|TSC1 antibody|Tsc1 gene antibody|TSC1_HUMAN antibody|Tuberous sclerosis 1 antibody|Tuberous sclerosis 1 protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Hamartin antibody, ASA-B0834–1.jpg
All lanes: Anti Hamartin (ASA-B0834) at 0.5ug/ml
WB: Recombinant Human Hamartin Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti Hamartin (ASA-B0834) at 0.5ug/ml
WB: Recombinant Human Hamartin Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-Hamartin antibody, ASA-B0834–2.jpg
All lanes: Anti Hamartin (ASA-B0834) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Hela Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
All lanes: Anti Hamartin (ASA-B0834) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Hela Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
