Description
Data Sheet
| Amino acid sequence | MSLTKTERTI IVSMWAKIST QADTIGTETL ERLFLSHPQT KTYFPHFDLH PGSAQLRAHG SKVVAAVGDA VKSIDDIGGA LSKLSELHAY ILRVDPVNFK LLSHCLLVTL AARFPADFTA EAHAAWDKFL SVVSSVLTEK YRLEHHHHHH. |
| Description | HBZ Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 150 amino acids (1-142 a.a.) and having a molecular mass of 16.7 kDa. The HBZ is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The HBZ solution contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl and 20% glycerol. |
| Protein Background | Hemoglobin subunit zeta (HBZ) is part of the globin family. Hemoglobin Zeta is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear colorless solution. |
| Reference | Western-Blot 0.1ug -1ug per strip, ELISA 1ug/Well. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | Zeta-globin, HBAZ, HBZ2, Hemoglobin subunit zeta, Hemoglobin zeta chain,HBZ. |
