Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| hexosaminidase A(alpha polypeptide) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human HEXA(191-207aa DVMAYNKLNVFHWHLVD), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HEXA |
Protein |
Beta-hexosaminidase subunit alpha |
Uniprot ID |
P06865 |
Function |
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. |
Tissue Specificity |
|
Sub-cellular localization |
Lysosome. |
Sequence Similarities |
Belongs to the glycosyl hydrolase 20 family. |
Aliases |
Beta hexosaminidase alpha chain precursor antibody|Beta hexosaminidase subunit alpha antibody|Beta N acetylhexosaminidase antibody|Beta N acetylhexosaminidase subunit alpha antibody|Beta-hexosaminidase subunit alpha antibody|Beta-N-acetylhexosaminidase subunit alpha antibody|Hexa antibody|HEXA_HUMAN antibody|Hexosaminidase A(alpha polypeptide) antibody|Hexosaminidase A alpha polypeptide antibody|Hexosaminidase A antibody|Hexosaminidase subunit A antibody|MGC99608 antibody|N acetyl beta glucosaminidase antibody|N acetyl beta glucosaminidase subunit alpha antibody|N-acetyl-beta-glucosaminidase subunit alpha antibody|TSD antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HEXA antibody, ASA-B0859, Western blotting
All lanes: Anti HEXA (ASA-B0859) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
All lanes: Anti HEXA (ASA-B0859) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
Anti-HEXA antibody, ASA-B0859, IHC(P)
IHC(P):Human Intestinal Cancer Tissue
IHC(P):Human Intestinal Cancer Tissue
