Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| hexosaminidase B (beta polypeptide) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human HEXB recombinant protein (Position: K381-M556). Human HEXB shares 75% and 73% amino acid (aa) sequences identity with mouse and rat HEXB, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HEXB |
Protein |
Beta-hexosaminidase subunit beta |
Uniprot ID |
P07686 |
Function |
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. |
Tissue Specificity |
|
Sub-cellular localization |
Lysosome. |
Sequence Similarities |
Belongs to the glycosyl hydrolase 20 family. |
Aliases |
Beta hexosaminidase beta chain antibody|Beta hexosaminidase subunit beta antibody|Beta N acetylhexosaminidase antibody|Beta-hexosaminidase subunit beta chain A antibody|Beta-N-acetylhexosaminidase subunit beta antibody|Cervical cancer proto oncogene 7 protein antibody|Cervical cancer proto-oncogene 7 protein antibody|ENC 1AS antibody|HCC 7 antibody|HCC-7 antibody|HCC7 antibody|HEX B antibody|Hexb antibody|HEXB_HUMAN antibody|Hexosaminidase B (beta polypeptide) antibody|Hexosaminidase B antibody|Hexosaminidase subunit B antibody|HexosaminidaseB antibody|N acetyl beta glucosaminidase antibody|N-acetyl-beta-glucosaminidase subunit beta antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HEXB antibody, ASA-B0860, Western blotting
All lanes: Anti HEXB (ASA-B0860) at 0.5ug/ml
WB: Recombinant Human HEXB Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti HEXB (ASA-B0860) at 0.5ug/ml
WB: Recombinant Human HEXB Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- HEXB antibody, ASA-B0860, Western blotting
All lanes: Anti HEXB (ASA-B0860) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 63KD
Observed bind size: 63KD
All lanes: Anti HEXB (ASA-B0860) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 63KD
Observed bind size: 63KD
Anti- HEXB antibody, ASA-B0860,IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
