Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| hypoxia inducible factor 3, alpha subunit |
Polyclonal |
IgG |
Rabbit |
Human, Rat |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3(497-514aa DDDFQLNASEQLPRAYHR), different from the related rat and mouse sequences by five amino acids. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
HIF3A |
Protein
|
Hypoxia-inducible factor 3-alpha |
Uniprot ID
|
Q9Y2N7 |
Function
|
Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed:11573933, PubMed:16126907, PubMed:19694616, PubMed:20416395, PubMed:21069422). Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity). |
Tissue Specificity
|
Expressed in vascular cells (at protein level) (PubMed:21069422). Expressed in kidney (PubMed:11573933, PubMed:19694616). Expressed in lung epithelial cells (PubMed:16775626). Expressed in endothelial cells (venous and arterial cells from umbilical cord and aortic endothelial cells) and in vascular smooth muscle cells (aorta) (PubMed:21069422). Strongly expressed in the heart, placenta, and skeletal muscle, whereas a weak expression profile was found in the lung, liver, and kidney (PubMed:12538644). Expressed weakly in cell renal cell carcinoma (CC-RCC) compared to normal renal cells (PubMed:16126907). Expression is down-regulated in numerous kidney tumor cells compared to non tumor kidney tissues (PubMed:16126907). Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas and in numerous cancer cell lines (PubMed:20416395). Isoform 3 and isoform 4 are weakly expressed in heart, placenta, lung, liver, skeletal muscle and pancreas (PubMed:20416395). Isoform 4 is expressed in fetal tissues, such as heart, brain, thymus, lung, liver, skeletal kidney and spleen (PubMed:20416395). Isoform 3 is weakly expressed in fetal tissues, such as liver and kidney (PubMed:20416395). |
Sub-cellular localization
|
Nucleus. |
Sequence Similarities
|
Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases
|
Basic-helix-loop-helix-PAS protein MOP7 antibody|bHLHe17 antibody|Class E basic helix-loop-helix protein 17 antibody|HIF 3A antibody|HIF 3A4 antibody|HIF-3-alpha antibody|HIF3 alpha antibody|HIF3-alpha antibody|HIF3-alpha-1 antibody|HIF3A antibody|HIF3A_HUMAN antibody|Hypoxia Inducible Factor 3 alpha antibody|Hypoxia inducible factor 3 alpha subunit antibody|Hypoxia inducible factor three alpha antibody|Hypoxia-inducible factor 3-alpha antibody|Inhibitory PAS domain protein antibody|IPAS antibody|Member of PAS protein 7 antibody|MOP7 antibody|PAS domain-containing protein 7 antibody|PASD7 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Rat Human |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HIF3 antibody, ASA-B0867, Western blotting
WB: Rat Brain Tissue Lysate
