Description
Kit Components
| HIV p24 standard (lyophilized) | 4 ng/vial ×2 |
| Detection antibody-HRP | 1 vial, dilution 1:60, 180 µL |
| 96-well plate pre-coated with anti-HIV p24 Ab | 1 |
| Sample Diluent buffer | 12 ml |
| Antibody diluent buffer | 12 ml |
| Chromogen Solution A (H2O2) | 6 ml |
| Chromogen Solution B (TMB) | 6 ml |
| Stop Solution | 6 ml |
| 20 × Wash Buffer | 25 ml |
Data Sheet
| Alternate Names | Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag) |
| Application | For quantitative determination of human HIV-1 p24. |
| Assay Range | 62.5 – 4000 pg/ml |
| Assay Time | 4.5 hours |
| Assay Type | Sandwich ELISA |
| Background | HIV-‐1 P24 is a 230aa phosphorylated protein of the Human Immunodeficiency Virus type 1 capsid forming the conical core of the virus that encapsulates the genomic RNA-‐nucleocapsid complex. P24 is a cleavage product of the Gag polyprotein (between aa132-‐133 and aa363-‐364) by viral proteases. HIV-‐1 P24 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. P24 is frequently used for HIV detection in blood, serum samples and other bodily fluids in acute HIV seroconversion, in neonatal infection and for monitoring of responses to antiviral drug therapy. P24 is also likely to be an integral part of any multi component HIV vaccine.
|
| Dilution Factors | Varies. Need to test before a large-scale assay. |
| Expiration | Six months at 2 – 8 ⁰C. |
| Gene ID | 155030 |
| Kit Type | Colorimetric ELISA |
| Precision | Intra-Assay 3.7%; Inter-Assay 7.9% |
| Recovery | 89 – 102% |
| Sample type | Culture Supernates, Serum, plasma, body fluids, tissue lysates. |
| Sensitivity | 10 pg/ml |
| Size | 96 T |
| Specificity | No significant cross-reactivity or interference between p24 and analogues was observed. |
| Storage | Storage of components varies. See protocol for specific instructions. |
| UniProt ID | P04591 |
