Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| major histocompatibility complex, class I, A | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human HLA A (82-106aa EYWDGETRKVKAHSQTHRVDLGTLR). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HLA-A |
Protein |
HLA class I histocompatibility antigen, A-2 alpha chain |
Uniprot ID |
P01892 |
Function |
Involved in the presentation of foreign antigens to the immune system. |
Tissue Specificity |
|
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Belongs to the MHC class I family. |
Aliases |
A 28 antibody|A 9 antibody|Antigen presenting molecule antibody|Aw 24 antibody|Aw 68 antibody|CLASS I HISTOCOMPATIBILITY ANTIGEN antibody|H2 K1 antibody|H2K antibody|HLA A antibody|HLA class I histocompatibility antigen A 1 alpha chain antibody|HLA class I histocompatibility antigen A 11 alpha chain antibody|HLA class I histocompatibility antigen A 2 alpha chain antibody|HLA class I histocompatibility antigen A 24 alpha chain antibody|HLA class I histocompatibility antigen A 3 alpha chain antibody|HLA class I histocompatibility antigen A 30 alpha chain antibody|HLA class I histocompatibility antigen A 32 alpha chain antibody|HLA class I histocompatibility antigen A 68 alpha chain antibody|HLA class I histocompatibility antigen, A 1 alpha chain antibody|HLAA antibody|Leukocyte antigen class I A antibody|Major histocompatibility complex class I A antibody|Major histocompatibility complex, class I, A antibody|MHC class I antigen A 1 antibody|MHC class I antigen A*11 antibody|MHC class I antigen A*2 antibody|MHC class I antigen A*24 antibody|MHC class I antigen A*3 antibody|MHC class I antigen A*30 antibody|MHC class I antigen A*32 antibody|MHC class I antigen A*68 antibody|MHC class I antigen HLA A heavy chain antibody|MHC class I heavy chain H2 K antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HLA-A antibody, ASA-B0871, Western blotting
All lanes: Anti HLA-A (ASA-B0871) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti HLA-A (ASA-B0871) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 41KD
Observed bind size: 41KD
Anti- HLA-A antibody, ASA-B0871,IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti- HLA-A antibody, ASA-B0871,IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
