HNF-4-alpha Antibody

HNF-4-alpha Antibody

$365.00

In stock

SKU: ASA-B0879 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
hepatocyte nuclear factor 4, alpha Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, IHC-F, WB Immunogen affinity purified.

Immunogen
E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequences identity with mouse and rat HNF-4-alpha, respectively.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 HNF4A

Protein

 Hepatocyte nuclear factor 4-alpha

Uniprot ID

 P41235

Function

 Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine.

Tissue Specificity

Sub-cellular localization

 Nucleus.

Sequence Similarities

 Belongs to the nuclear hormone receptor family. NR2 subfamily.

Aliases

 FLJ39654 antibody|Hepatic nuclear factor 4 alpha antibody|Hepatocyte nuclear factor 4 alpha antibody|Hepatocyte nuclear factor 4 antibody|Hepatocyte nuclear factor 4-alpha antibody|HNF 4 alpha antibody|HNF 4 antibody|HNF-4-alpha antibody|HNF4 antibody|HNF4 antibody|HNF4A antibody|HNF4A_HUMAN antibody|HNF4a7 antibody|HNF4a8 antibody|HNF4a9 antibody|Hnf4alpha antibody|HNF4alpha10/11/12 antibody|MODY 1 antibody|MODY antibody|MODY1 antibody|NR2A1 antibody|NR2A1 antibody|NR2A21 antibody|Nuclear receptor subfamily 2 group A member 1 antibody|OTTHUMP00000031060 antibody|OTTHUMP00000031062 antibody|TCF 14 antibody|TCF antibody|TCF-14 antibody|TCF14 antibody|TCF14 antibody|Tcf4 antibody|Transcription factor 14, hepatic nuclear factor antibody|Transcription factor 14 antibody|Transcription factor HNF 4 antibody|Transcription factor HNF-4 antibody|Transcription factor HNF4 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat AssaySolutio’s IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Mouse, Rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- HNF-4-alpha antibody, ASA-B0879, Western blotting
All lanes: Anti HNF-4-alpha (ASA-B0879) at 0.5ug/ml
WB: Recombinant Human HNF-4-alpha Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- HNF-4-alpha antibody, ASA-B0879, Western blotting
All lanes: Anti HNF-4-alpha (ASA-B0879) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
Anti- HNF-4-alpha antibody, ASA-B0879, IHC(P)
IHC(P): Rat Kidney Tissue

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