Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| one cut homeobox 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human HNF6(187-203aa LGSIHNSQQGLPHYAHP), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ONECUT1 |
Protein |
Hepatocyte nuclear factor 6 |
Uniprot ID |
Q9UBC0 |
Function |
Transcriptional activator. Binds the consensus sequence 5′-DHWATTGAYTWWD-3′ on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. |
Tissue Specificity |
Highly expressed in liver; lower expression in testis and skin. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the CUT homeobox family. |
Aliases |
Hepatocyte nuclear factor 6 antibody|HNF 6 antibody|HNF6A antibody|One cut domain family member 1 antibody|Onecut1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HNF6 antibody, ASA-B0883, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Mouse Liver Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Mouse Liver Tissue Lysate
Lane 3: HELA Cell Lysate
Anti-HNF6 antibody, ASA-B0883, IHC(P)
IHC(P): Human Liver Cancer Tissue
IHC(P): Human Liver Cancer Tissue
Anti-HNF6 antibody, ASA-B0883, IHC(P)
IHC(P): Rat Liver Tissue
IHC(P): Rat Liver Tissue
