Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| homeobox A10 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human HOXA10 recombinant protein (Position: Y57-R340). Human HOXA10 shares 87% amino acid (aa) sequence identity with mouse HOXA10. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HOXA10 |
Protein |
Homeobox protein Hox-A10 |
Uniprot ID |
P31260 |
Function |
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to the DNA sequence 5′-AA[AT]TTTTATTAC-3′. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the Abd-B homeobox family. |
Aliases |
centaurin alpha 1 like antibody|homeo box A10 antibody|Homeobox A10 antibody|homeobox protein 1H antibody|Homeobox protein Hox A10 antibody|Homeobox protein Hox-1.8 antibody|Homeobox protein Hox-1H antibody|Homeobox protein Hox-A10 antibody|homeobox protein HOXA10 antibody|HOX 1 antibody|HOX 1.8 antibody|HOX 1H antibody|HOX A10 antibody|HOX1 antibody|HOX1.8 antibody|HOX1H antibody|HOXA 10 antibody|HOXA10 antibody|HOXA10 protein antibody|HXA10_HUMAN antibody|MGC12859 antibody|PL antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HOXA10 antibody, ASA-B0886, Western blotting
All lanes: Anti HOXA10 (ASA-B0886) at 0.5ug/ml
WB: Recombinant Human HOXA10 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti HOXA10 (ASA-B0886) at 0.5ug/ml
WB: Recombinant Human HOXA10 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- HOXA10 antibody, ASA-B0886, Western blotting
All lanes: Anti HOXA10 (ASA-B0886) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti HOXA10 (ASA-B0886) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
