Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| homeobox A4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human HOXA4(9-26aa NSNYIEPKFPPFEEYAQH), different from the related mouse sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HOXA4 |
Protein |
Homeobox protein Hox-A4 |
Uniprot ID |
Q00056 |
Function |
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5′-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5′-TAATGA[CG]-3′ and 5′-CTAATTTT- 3′. |
Tissue Specificity |
Embryonic nervous system. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the Antp homeobox family. Deformed subfamily. |
Aliases |
Dfd like protein antibody|Homeo box A4 antibody|Homeobox A4 antibody|Homeobox protein Hox-1.4 antibody|Homeobox protein Hox-1D antibody|Homeobox protein Hox-A4 antibody|Hox 1.4 like protein antibody|hox-1.4 antibody|hox-1d antibody|HOX1 antibody|HOX1D antibody|HOXA4 antibody|HXA4_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HOXA4 antibody, ASA-B0889, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: PC-12 Cell Nuclear Lysate
Lane 1: SW620 Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: PC-12 Cell Nuclear Lysate
Anti-HOXA4 antibody, ASA-B0889, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-HOXA4 antibody, ASA-B0889, ICC
ICC: HELA Cell
ICC: HELA Cell
