Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| hydroxysteroid(17-beta) dehydrogenase 2 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence at the C-terminus of mouse HSD17B2(274-290aa QDIWDKMEKEILDHFSK), different from the related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HSD17B2 |
Protein |
Estradiol 17-beta-dehydrogenase 2 |
Uniprot ID |
P51658 |
Function |
Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH (By similarity). |
Tissue Specificity |
|
Sub-cellular localization |
Membrane ; Single-pass type II membrane protein . |
Sequence Similarities |
|
Aliases |
17 beta HSD 2 antibody|17-beta-HSD 2 antibody|17-beta-hydroxysteroid dehydrogenase type 2 antibody|20 alpha HSD antibody|20 alpha hydroxysteroid dehydrogenase antibody|20 alpha-hydroxysteroid dehydrogenase antibody|20-alpha-HSD antibody|DHB2_HUMAN antibody|E2DH antibody|EDH17B2 antibody|Estradiol 17-beta-dehydrogenase 2 antibody|HSD17 antibody|Hsd17b2 antibody|Hydroxysteroid(17 beta) dehydrogenase 2 antibody|Microsomal 17 beta hydroxysteroid dehydrogenase antibody|Microsomal 17-beta-hydroxysteroid dehydrogenase antibody|SDR9C2 antibody|Testosterone 17 beta dehydrogenase antibody|Testosterone 17-beta-dehydrogenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSD17B2 antibody, ASA-B0903, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
Anti-HSD17B2 antibody, ASA-B0903, Western blotting
WB: Rat Liver Tissue Lysate
WB: Rat Liver Tissue Lysate
