Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| heat shock transcription factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human HSF1(380-397aa DKNELSDHLDAMDSNLDN), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HSF1 |
Protein |
Heat shock factor protein 1(HSF 1) |
Uniprot ID |
Q00613 |
Function |
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. Nucleus. Note: Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules. |
Sequence Similarities |
|
Aliases |
Heat shock factor 1 antibody|Heat shock factor protein 1 antibody|Heat shock transcription factor 1 antibody|HSF 1 antibody|hsf1 antibody|HSF1_HUMAN antibody|HSTF 1 antibody|HSTF1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSF1 antibody, ASA-B0906, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: MCF-7 Cell Lysate
Anti-HSF1 antibody, ASA-B0906, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
