Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| DnaJ(Hsp40) homolog, subfamily B, member 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp40(317-332aa EFEVIFPERIPQTSRT), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DNAJB1 |
Protein |
DnaJ homolog subfamily B member 1 |
Uniprot ID |
P25685 |
Function |
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Contains 1 J domain. |
Aliases |
DnaJ(Hsp40) homolog subfmaily B member 1 antibody|DNAJ 1 antibody|DNAJ B1 antibody|DnaJ homolog subfamily B member 1 antibody|DnaJ protein homolog 1 antibody|DNAJ1 antibody|DNAJB 1 antibody|DNAJB1 antibody|DNAJB1 protein antibody|DNJB1_HUMAN antibody|HDJ 1 antibody|HDJ-1 antibody|HDJ1 antibody|Heat shock 40 kDa protein 1 antibody|Heat shock 40kD protein 1 antibody|Heat shock protein 40 antibody|Hsp 40 antibody|Hsp40 antibody|HSPF 1 antibody|HSPF1 antibody|Human DnaJ protein 1 antibody|Radial spoke 16 homolog B antibody|RSPH16B antibody|Sis1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Hsp40 antibody, ASA-B0913, Western blotting
WB: HELA Cell Lysate
WB: HELA Cell Lysate
Anti-Hsp40 antibody, ASA-B0913, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
