Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| heat shock protein 90kDa alpha(cytosolic), class A member 1; heat shock protein 90kDa alpha(cytosolic), class B member 1 |
Monoclonal |
IgG |
Mouse |
Human, Mouse, Rabbit, Rat |
WB |
Ascites |
Immunogen
|
| Heat shock protein 90(HSP90) from the water mold Achlya ambisexualis. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
HSP90AA1; HSP90AB1 |
Protein
|
Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-beta |
Uniprot ID
|
Human(P07900), Mouse(P07901), Rat(P82995), Human(P08238), Mouse(P11499), Rat(P34058) |
Function
|
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. |
Tissue Specificity
|
|
Sub-cellular localization
|
Cytoplasm. Melanosome. Cell membrane. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Sequence Similarities
|
|
Aliases
|
HSP90A|HSPC1|HSPCA|HSP90B|HSPC2|HSPCB |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
2-4μg/ml |
Human, mouse, rabbit, rat |
AssaySolutio’s ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
