Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| HSPB(heat shock 27kDa) associated protein 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human HSPBAP1(118-136aa YDHSKFWAYADYKYFVSLF), different from the related rat sequences by one amino acid, and different from the related mouse sequences by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HSPBAP1 |
Protein |
HSPB1-associated protein 1 |
Uniprot ID |
Q96EW2 |
Function |
May play a role in cellular stress response. |
Tissue Specificity |
Widely expressed. |
Sub-cellular localization |
Cytoplasm . |
Sequence Similarities |
Contains 1 JmjC domain. |
Aliases |
27 KdA heat shock protein-associated protein 1 antibody|HBAP1_HUMAN antibody|HSPB1-associated protein 1 antibody|HSPBAP1 antibody|PASS1 antibody|Protein associated with small stress protein 1 antibody|protein associating with small stress protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HSPBAP1 antibody, ASA-B0930, Western blotting
All lanes: Anti HSPBAP1 (ASA-B0930) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 55 KD
Observed bind size: 55 KD
All lanes: Anti HSPBAP1 (ASA-B0930) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 55 KD
Observed bind size: 55 KD
