Description
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant GLA standard: 10ng/vial – 2.
2. One 96-well plate precoated with anti-GLA antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-GLA antibody: 1 vial, dilution 1:2000.
5. Detection Antibody diluention buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. Defects in GLA are the cause of Fabry disease (FD).
Data Sheet
| Application | For quantitative determination of Human alphaGalactosidase A / GLA. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Assay Range | 125 – 8000 pg/ml |
| Expiration | Six months at 2 – 8 ⁰C. |
| Sample type | Culture Supernates, Serum, plasma, body fluids, tissue lysates. |
| Sensitivity | 100 pg/ml |
| Size | 96T |
| Storage | Store at 2 – 8⁰C. Keep Standard and detection antibody at -20⁰C. |
