Description
|
Assay Range |
62.5–4000 pg/mL |
|
Sensitivity |
10.0 pg/mL |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample volume |
100 µL final volume, dilution factor varies on samples |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Human B7-1 standard: 2 vials
2. One 96-well plate precoated with anti-Human B7-1 Ab
3. Sample diluent buffer: 12 mL – 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
B7-1, also known as T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, or CD80, is a type I transmembrane protein belonging to the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 shares 26% aa identity with its homolog and partner human B7-2. Human B7-1 is 44% aa identical to its mouse counterpart. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on inter-digitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.
B7-1 and B7-2 are the ligands for CD28 and CTLA4. They constitute one of the dominant costimulatory pathways that regulate T and B cell responses and are essential for T cell activation and survival. CTLA4 binds to B7-1 and B7-2 with a much higher affinity than CD28 and is involved in the down-regulation of the immune response.
