Description
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant BST1 standard: 1ng/vial – 2.
2. One 96-well plate precoated with anti-BST1 antibody.
3. Sample diluent buffer: 12 ml
4. Biotin conjugated anti-BST1 antibody: 1 vial, dilution 1:1500.
5. Detection Antibody diluention buffer: 12ml-2.
6. Streptavidin-HRP: 10µL, dilution 1:2000.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Bone marrow stromal cell antigen-1 is a stromal cell line-derived lycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. The murine CD157 is a useful marker for lymphoid progenitor cells initiating gene rearrangement of their antigen receptors.
Data Sheet
| Application | For quantitative determination of Human BST1. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Assay Range | 15.6 – 1000 pg/ml |
| Expiration | Six months at 2 – 8 ⁰C. |
| Sensitivity | 10 pg/ml |
| Size | 96 T |
| Storage | Store at 2 – 8⁰C. Keep Standard and detection antibody at -20⁰C. |
