Description
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CPB1 standard: 2 ng/vial – 2.
2. One 96-well plate precoated with anti-human CPB1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CPB1 Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. Assay diluent buffer: 12 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg It is not elevated in pancreatic carcinoma.
Data Sheet
| Assay Range | 6.25 – 400 pg/ml |
