Description
Assay Format
Sandwich ELISA.
Storage
Store at 4 °C.
Expiration
12 months at 4 °C.
Range
9.4 pg/mL – 600 pg/mL.
Sensitivity
5 pg/mL.
Specificity
No detectable cross-reactivity with any other cytokine.
Application
cell culture supernatants, serum and plasma.
Kit Components
1. Lyophilized recombinant Human CCL2 standard: 2 ng/tube-2.
2. One 96-well plate precoated with anti-Human CCL2 antibody.
3. Sample diluent buffer: 12 mL -2
4. Biotinylated anti-Human CCL2 antibody: 60µL, dilution 1:200.
5. Antibody diluent buffer: 12ml.
6. Streptavidin-HRP: 60µL, dilution 1:200.
7. Streptavidin-HRP diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Data Sheet
| Assay Range | 9.4 – 600pg/ml |
