Description
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CCN3 standard: 10ng/vial – 2.
2. One 96-well plate precoated with anti-human CCN3 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CCN3 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. Assay diluent buffer: 12 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
NOV (nephroblastoma overexpressed) also known as CCN3 is a matricellular protein. It is a matricellular protein of the CCN family, which also includes CCN1 (CYR61), CCN2 (CTGF), CCN4 (WISP-1), CCN5 (WISP-2), and CCN6 (WISP-3). During development, CCN3 is expressed widely in derivatives of all three germ layers, and high levels of expression are observed in smooth muscle cells of the arterial vessel wall. Altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm’s tumors, Ewing’s sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas.
Data Sheet
| Assay Range | 31.25 – 2000 pg/ml |
