Description
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD300C standard: 0.6 ng/vial – 2.
2. One 96-well plate precoated with anti-human CD300C Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD300C Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CMRF35-like molecule 6 (CLM-6) also known as CD300 antigen-like family member C (CD300c) is a single-pass type I membrane protein which belongs to theCD300 family. The CD300, alternately designated the LMIR family, contains six to eight type I membrane proteins expressed mainly on cells of the myeloid lineage. Each has a single extracellular V-type Ig-like domain. Some have cytoplasmic ITIM motifs that result in the delivery of an inhibitory signal; others can interact with ITAM motifs and function as activating receptors. Human CD300 proteins have also been designated IREM (immune receptor expressed by myeloid cells) or CMRF-35 for the original identifying antibody.
Data Sheet
| Assay Range | 9.4 – 600 pg/ml |
