Description
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD89 standard: 2 ng/vial – 2.
2. One 96-well plate precoated with anti-human CD89 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD89 Ab: 1 vial, dilution 1:250.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Fragment of IgA receptor (FCAR) also known as CD89 (Cluster of Differentiation89), is a member of the immunoglobulin superfamily. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators.
Data Sheet
| Assay Range | 7.8 – 500 pg/ml |
