Description
|
Assay Range |
39 -40,000 pg/mL |
|
Sensitivity |
30.0 pg/mL |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample volume |
100 µL final volume, dilution factor varies on samples. |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Human C1q standard: 2 vials.
2. One 96-well plate precoated with anti- Human C1q Ab
3. Diluent buffer (10x): 30 mL x1
4. Detection antibody: 140 µLx1
5. Streptavidin-HRP (100x): 80 µL x 1
6. TMB color developing agent : 8 mL x1.
7. TMB stop solution: 12 mL x1.
8. Washing solution (20x): 30 mL x2.
Background
C1q is a part and the recognition subunit of the C1 complex composed of three different proteins (C1q, C1r, and C1s) bound together in a calcium-dependent complex. The C1 complex is the first complement component in the classical pathway of complement. C1q is a 460 kDa protein comprising six heterotrimeric collagen-like triple helices. The globular heads of the C1q bind to the Fc-fragment of IgM or IgG on the surface of a pathogen, playing an important role in host defense and apoptotic cell clearance. It is a functional ligand for leukocyte-associated Ig-like receptor 1 restricting immune cell differentiation and activation. C1q prevents toxicity induced by oligomeric forms of amyloid-β. Failure to efficiently clear apoptotic cells in the absence of C1q is associated with lupus-like autoimmunity.
