Description
|
Assay Range |
234 – 15,000 pg/mL |
|
Sensitivity |
200 pg/mL |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample volume |
50 µL final volume, dilution factor varies on samples. |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Human C9 standard: 1 vial.
2. One 96-well plate precoated with anti- Human C9 Ab
3. Diluent buffer (10x): 30 mL x1
4. Detection antibody: 1 vial
5. Streptavidin-HRP (100x): 80 µL x 1
6. TMB color developing agent : 8 mL x1.
7. TMB stop solution: 12 mL x1.
8. Washing solution (20x): 30 mL x2.
Background
Human C9 is a 537 amino acid (aa) single-chain glycosylated (7.8%) protein with a molecular weight of 71KDa. C9 binds to the C5b-8 complex and forms the mature membrane attack complex (MAC) on cell membranes. The protease α-thrombin cleaves C9 at 294 aa residues from the carboxy-terminal end and produces two single-chain polypeptides: a hydrophilic C9a and a hydrophobic C9b. In the presence of membrane bound components C5b-8, C9 inserts into the phopholipid bilayer and becomes a pore-forming subunit of the membrane attack complex (MAC) on target membranes. C9 deficiency has been associated with a significantly increased risk of developing meningococcal meningitis.
