Description
Human Copeptin ELISA Kit
Target Introduction
Copeptin (also known as CT-proAVP) is a 39-amino acid-long peptide derived from the C-terminus of pre-pro-hormone of arginine vasopressin, neurophysin II and copeptin. Arginine vasopressin (AVP), also known as the antidiuretic hormone (ADH), is involved in multiple cardiovascular and renal pathways and abnormal level of AVP are associated with various diseases. Hence measurement of AVP would be useful, but is not commonly carried out in clinical practice because of its very short half-life making it difficult to quantify. In contrast, copeptin can be immunologically tested with ease and therefore can be used as a vasopressin surrogate marker
Copeptin is a 39-amino acid-long, glycosylated peptide. It is synthesized mainly in the paraventricular neurons of the hypothalamus and in the supraoptical nucleus. During axonal transport, pre-pro-AVP is proteolytically cleaved into vasopressin, neurophysin II and copeptin. These molecules are then stored in secretory granules in the posterior pituitary and released upon osmotic or non-osmotic (hemodynamical; stress-related) stimuli.
The size and half-life of copeptin permit an easier immunological testing, compared to vasopressin, and hence copeptin is proposed as a reliable AVP surrogate. The clinical interest in copeptin testing is closely linked to the pathophysiological pathways in which vasopressin is involved: polydipsia-polyuria syndrome, hyponatremia, syndrome of inappropriate antidiuretic hormone secretion (SIADH) as well as heart failure and acute coronary syndrome.
The concentration of copeptin in the blood circulation ranges from 1 to 12 pmol/L ( 5 to 60 pg/mL) in healthy individuals. The levels of copeptin are slightly higher in men than in women and are not influenced by age. In response to serum osmolality fluctuations, the kinetics of copeptin are comparable to those of vasopressin. For example, patients with an electrolyte disorders such as diabetes insipidus with very low vasopressin concentrations also show very low copeptin concentrations in blood plasma. On the other hand, patients suffering from syndrome of inappropriate antidiuretic hormone secretion show high concentrations of both vasopressin and copeptin.
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Different lot may vary, please follow the instruction coming with the kit.
Data Sheet
| Alternate Names | copeptine, CT-proAVP |
| Assay Range | 62.5 to 4000 pg/mL |
| Assay Time | 4.5 hours |
| Assay Type | Sandwich ELISA |
| Background | Copeptin (also known as CT-proAVP) is a 39-amino acid-long peptide derived from the C-terminus of pre-pro-hormone of arginine vasopressin, neurophysin II and copeptin. Arginine vasopressin (AVP), also known as the antidiuretic hormone (ADH), is involved in multiple cardiovascular and renal pathways and abnormal level of AVP are associated with various diseases. Hence measurement of AVP would be useful, but is not commonly carried out in clinical practice because of its very short half-life making it difficult to quantify. In contrast, copeptin can be immunologically tested with ease and therefore can be used as a vasopressin surrogate marker. The concentration of copeptin in the blood circulation ranges from 1 to 12 pmol/L ( 5 to 60 pg/mL) in healthy individuals. The levels of copeptin are slightly higher in men than in women and are not influenced by age. In response to serum osmolality fluctuations, the kinetics of copeptin are comparable to those of vasopressin. For example, patients with an electrolyte disorders such as diabetes insipidus with very low vasopressin concentrations also show very low copeptin concentrations in blood plasma. On the other hand, patients suffering from syndrome of inappropriate antidiuretic hormone secretion show high concentrations of both vasopressin and copeptin. |
| Immunogen/Antigen | synthetic and native copeptin peptide |
| Kit Type | Colorimetric |
| Precision | Inter-assay– less than 7.8%; Intra-assay– less than 3.6% |
| Recovery | 95.3% – 108% |
| Sample Type | Serum |
| Sensitivity | 20.6 pg/mL |
| Specificity | Only recognize synthetic and native copeptin peptide |
| UniProt ID | P01185 |
