Description
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human COQ7 standard: 5 ng/vial – 2.
2. One 96-well plate precoated with anti-human COQ7 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human COQ7 Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
COQ7 (Coenzyme Q biosynthesis protein 7 homolog) is a mitochondrion inner membrane and peripheral membrane protein which belongs to theCOQ7 family. COQ7 is potential central metabolic regulator involved in lifespan determination in ubiquinone-independent manner. It is expressed dominantly in heart and skeletal muscle.
Data Sheet
| Assay Range | 39.1 – 2500 pg/ml |
