Description
Sensitivity
1.5 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human EPOR standard: 2.5 ng/vial – 2.
2. One 96-well plate precoated with anti-human EPOR Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human EPOR Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The erythropoietin receptor (EpoR) belongs to the type I cytokine receptor family and is produced by kidney and liver in an oxygen-dependent manner. It mediates erythropoietin-induced erythroblast proliferation and differentiation. The stimulated EPOR appears to function in erythroid cell survival through preventing the PCD, and is also suggested to play a neuroprotective role for its expression in mammalian brains.
Data Sheet
| Assay Range | 1.95 – 125 pg/ml |
