Description
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human HGFA standard: 5 ng/vial – 2.
2. One 96-well plate precoated with anti-human HGFA Ab
3. Sample diluent buffer: 12 mL x 1
4. Biotin-conjugated anti-Human HGFA Ab: 1 vial.
5. Detection Antibody diluent buffer: 12 mL x 2
6. Streptavidin-HRP: 1 vial, 1:200 dilution.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Hepatocyte growth factor activator, also known as HGF activator, HGFA, is a serum-derived serine protease which belongs to the peptidase family S1. HGFA is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. HGF activator (HGFA) is a serine protease that cleaves the single-chain HGF precursor, generating the active heterodimer. HGFA is secreted by the liver and normally circulates in the blood as an inactive zymogen, which can be activated by thrombin. The active protease is regulated by HGFA inhibitors.
Data Sheet
| Assay Range | 39 – 2500 pg/ml |
