Description
Product name
Human-1alpha ELISA Kit
Catalog No.
NR-E10234
Size
96T
Range
7.8 pg/mL-500 pg/mL
Sensitivity
3.1 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 °C for frequent use, at -20 °C for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
6 months at 4 °C and 12 months at -20 °C.
Application
serum, plasma, cell culture supernates.
Kit Components
1. Lyophilized recombinant human IL-1a standard: 500 pg/tube-2.
2. One 96-well plate precoated with anti-human IL-1a antibody.
3. Sample diluent buffer: 12 ml x 2
4. Biotinylated anti-human IL-1a antibody: 60 µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Strepavidin-HRP: 300µL, dilution 1:40.
7. Strepavidin-HRPdiluent buffer: 12ml.
8. Chromogen-A, 6 ml; Chromogen-B, 6 ml.
9. Stop Solution: 6 ml.
Data Sheet
| Alternative Name of Analyte | IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha |
| Assay Range | 7.81-500pg/ml |
| Assay Time | 4.5 hours |
| Assay Type | Sandwich ELISA |
| Background | Interleukin 1α is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.
IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. |
| Gene ID | 3552 |
| Kit Type | Colorimetric ELISA |
| UniProt ID | P01583 |
