Description
Sensitivity
15 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human IL-33 standard: 1 ng/vial — 2.
2. One 96-well plate precoated with anti-human IL-33 Ab
3. Sample diluent buffer: 12 mL x 1
4. Biotin-conjugated anti-Human IL-33 Ab: 1 vial.
5. Detection Antibody diluent buffer: 12 mL x 2
6. Streptavidin-HRP: 1 vial, 1:200 dilution.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. IL-33 is found to be expressed in inflammatory diseases, including severe asthma and inflammatory bowl disease. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells.
Data Sheet
| Assay Range | 15.6 – 1000 pg/ml |
