Description
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human LCN1 standard: 2 ng/vial – 2.
2. One 96-well plate precoated with anti-human LCN1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human LCN1 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Lipocalin-1 is a secreted protein which belongs to the calycin superfamily and Lipocalin family. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. It may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren’s syndrome.
Data Sheet
| Assay Range | 31.25 – 2000 pg/ml |
