Description
The kit is used to quantify the human OSF-2 in cell culture supernatant, serum, plasma and other suitable sample solution.
FOR RESEARCH USE ONLY
Data Sheet
| Alternate Names | OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52 |
| Assay principle | The human OSF-2 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Rat anti-human OSF-2 specific antibody has been pre-coated onto 96-well plate. Human OSF-2 present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated goat anti-human OSF-2 detection antibody is added to form an Ab-Ag-Ab sandwich. Streptavidin-HRP is added and unbound conjugate is removed with wash Buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of human OSF-2 captured on plate. |
| Assay Range | 62.5 – 4000 pg/mL |
| Assay Time | 4.5 hours |
| Assay Type | Sandwich ELISA |
| Background | Periostin (POSTN, PN, or osteoblast-specific factor OSF-2) is a protein that in humans is encoded by the POSTN gene. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. Periostin is a gla domain vitamin K dependent factor.
Periostin is a secreted extracellular matrix protein that was originally identified in cells from the mesenchymal lineage (osteoblasts, osteoblast-derived cells, the periodontal ligament, and periosteum). It has been associated with the epithelial-mesenchymal transition in cancer and with the differentiation of mesenchyme in the developing heart. This protein shares a homology with fasciclin I, a secreted cell adhesion molecule found in insects. In many cancers, periostin binds to integrins on cancer cells, activating the Akt/PKB- and FAK-mediated signaling pathways. This leads to increased cell survival, invasion, angiogenesis, metastasis, and the epithelial-mesenchymal transition. In humans and mice, periostin undergoes alternative splicing in its C-terminal region, resulting in specific isoforms that can be observed in a broad range of cancers such as pancreatic, colon, and breast cancer. |
| Gene ID | 10631 |
| Kit Type | Colorimetric ELISA |
| Sample Type | Cell culture medium, Serum, Plasma |
| Sample Volume | 100 uL |
| Sensitivity | 43.2 pg/mL |
| Specificity | Only recognize native and recombinant OSF-2/Periostin. |
| Storage | Please refer to the manual. |
| UniProt ID | Q15063 |
