Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| KIT ligand |
Polyclonal |
IgG |
Rabbit |
Human |
ELISA, WB |
Immunogen affinity purified. |
Immunogen
|
| E. coli-derived human SCF recombinant protein(Position: E26-A190). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
KITLG |
Protein
|
Kit ligand |
Uniprot ID
|
P21583 |
Function
|
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
Tissue Specificity
|
|
Sub-cellular localization
|
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities
|
Belongs to the SCF family. |
Aliases
|
C kit ligand antibody|C-kit ligand antibody|Ckit ligand antibody|DKFZp686F2250 antibody|familial progressive hyperpigmentation 2 antibody|FPH2 antibody|KIT ligand antibody|Kitl antibody|KITLG antibody|KL 1 antibody|KL1 antibody|Mast cell growth factor antibody|MGF antibody|SCF antibody|SF antibody|SHEP7 antibody|sKITLG antibody|Soluble KIT ligand antibody|Steel factor antibody|Stem cell factor antibody|Stem cell factor precursor antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
| ELISA |
0.1-0.5μg/ml |
Human |
Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
