Description
Sensitivity
90 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human SPARCL1 standard: 10 ng/vial – 2.
2. One 96-well plate precoated with anti-human SPARCL1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human SPARCL1 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
SPARC-L1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as Hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins. SPARC-L1 is a widely expressed protein with antiadhesive properties and involves in many physiological functions. SPARC-L1 binds collagen and contributes to formation of dermal collagen fibrils. SPARC-L1 modulates the structure of dermal extracellular matrix, specifically by its regulation of decorin levels and collagen fibril assembly.
Data Sheet
| Assay Range | 93.75 – 6000 pg/ml |
