Description
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human TIM3 standard: 5 ng/vial – 2.
2. One 96-well plate precoated with anti-human TIM3 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human TIM3 Ab: 1 vial, dilution 1:250.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The T cell immunoglobulin and mucin domains (Tim) are a group of cell surface receptors differentially expressed on mature T cells and macrophages that can control the functionality of T cell subsets by inducing activating or apoptotic signals after interaction with specific ligands. Specifically, Tim3 is preferentially expressed on differentiated TH-1 cells, but also on cytotoxic CD8+ T cells, TH-17 cells, CD4+CD25+ regulatory T cells (Tregs), dendritic cells, and mast cells. Tim3 acts as a negative regulator of pro-inflammatory immune effector pathways.
Data Sheet
| Assay Range | 31.25 – 2000 pg/ml |
