Description
Sensitivity
6 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human Trk-B standard: 1 ng/vial – 2.
2. One 96-well plate precoated with anti-human Trk-B Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human Trk-B Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor is the high affinity catalytic receptor for several neurotrophins. TrkB mediates the multiple effects of these neurotrophic factors, which includes neuronal differentiation and survival.
Data Sheet
| Assay Range | 6.25 – 400 pg/ml |
